The noise and vibration characteristics play a vital role in the effectiveness of engine operations and performance of internal combustion engines. Accumulation of the higher amplitude of both noise and vibration affects the comfort of the engine. So far, most of the research done on the performance, combustion, and emission characteristics only. Less importance is shown in the form engine vibration and sounds created by the engine operation. This paper presents and explores the importance and experimental results of noise and vibration by the compression ignition diesel engine with the fuels of diesel and microalgae biodiesel. The produced microalgae biodiesel blends were SMB10%, SMB20%, and SMB30%. The experimental results were conducted at different engine loads varying across 25%, 50%, 75%, and 100%. The inline, four-cylinder, water-cooled, and naturally aspirated DI diesel engine was used as an experimental setup. From the comparative results between the diesel and microalgae biodiesel, it is found that the use of microalgae blended biodiesel reduced the noise and vibration. The higher the percentage of blends, the greater the reduction in sound and vibration will be. Apart from possessing the performance and emission qualities, the microalgae biodiesel blends proved to be an efficient fuel in reduced vibration and noise qualities too. In three directions, the vibrations were measured as lateral, longitudinal, and vertical vibrations. The vibration in the lateral direction was significantly reduced. Compelling the results, it is understood that the use of the microalgae blends can be sustainable from the perspective of engine wear and tear.