The in vitro culture process via bioreactors is critical to create tissue-engineered constructs (TECs) to repair or replace the damaged tissues/organs in various engineered applications. In the past, the TEC culture process was typically treated as a black box and performed on the basis of trial and error. Recently, computational fluid dynamics (CFD) has demonstrated its potential to analyze the fluid flow inside and around the TECs, therefore, being able to provide insight into the culture process, such as information on the velocity field and shear stress distribution that can significantly affect such cellular activities as cell viability and proliferation during the culture process. This paper briefly reviews the CFD and experimental methods used to investigate the in vitro culture process of skeletal-type TECs in bioreactors, where mechanical deformation of the TEC can be ignored. Specifically, this paper presents CFD modeling approaches for the analysis of the velocity and shear stress fields, mass transfer, and cell growth during the culture process and also describes various particle image velocimetry (PIV) based experimental methods to measure the velocity and shear stress in the in vitro culture process. Some key issues and challenges are also identified and discussed along with recommendations for future research.
Skip Nav Destination
Article navigation
December 2012
Research-Article
Investigation of the In Vitro Culture Process for Skeletal-Tissue-Engineered Constructs Using Computational Fluid Dynamics and Experimental Methods
Md. Shakhawath Hossain,
Md. Shakhawath Hossain
1
e-mail: mdh511@mail.usask.ca
1Corresponding author.
Search for other works by this author on:
D. J. Bergstrom
D. J. Bergstrom
e-mail: don.bergstrom@usask.ca
Department of Mechanical Engineering,
Department of Mechanical Engineering,
University of Saskatchewan
,57 Campus Drive Saskatoon, SK
,S7N 5A9
, Canada
Search for other works by this author on:
Md. Shakhawath Hossain
e-mail: mdh511@mail.usask.ca
X. B. Chen
e-mail: xbc719@mail.usask.ca
D. J. Bergstrom
e-mail: don.bergstrom@usask.ca
Department of Mechanical Engineering,
Department of Mechanical Engineering,
University of Saskatchewan
,57 Campus Drive Saskatoon, SK
,S7N 5A9
, Canada
1Corresponding author.
Contributed by the Bioengineering Division of ASME for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received May 23, 2012; final manuscript received October 4, 2012; accepted manuscript posted October 25, 2012; published online November 27, 2012. Assoc. Editor: Pasquale Vena.
J Biomech Eng. Dec 2012, 134(12): 121003 (11 pages)
Published Online: November 27, 2012
Article history
Received:
May 23, 2012
Revision Received:
October 4, 2012
Accepted:
October 25, 2012
Citation
Hossain, M. S., Chen, X. B., and Bergstrom, D. J. (November 27, 2012). "Investigation of the In Vitro Culture Process for Skeletal-Tissue-Engineered Constructs Using Computational Fluid Dynamics and Experimental Methods." ASME. J Biomech Eng. December 2012; 134(12): 121003. https://doi.org/10.1115/1.4007952
Download citation file:
Get Email Alerts
Cited By
Evaluation of an Unloading Concept for Knee Osteoarthritis: A Pilot Study in a Small Patient Group
J Biomech Eng (January 2024)
Related Articles
Design of an Ex Vivo Culture System to Investigate the Effects of Shear Stress on Cardiovascular Tissue
J Biomech Eng (June,2008)
Response of Arteries to Near-Wall Fluid Dynamic Behavior
Appl. Mech. Rev (May,1990)
Identifying Suitable Three-Dimensional Bio-Printed Scaffold Architectures to Incubate in a Perfusion Bioreactor: Simulation and Experimental Approaches
J. Med. Devices (June,2023)
Mass Transport in a Microchannel Bioreactor With a Porous Wall
J Biomech Eng (June,2010)
Related Proceedings Papers
Related Chapters
Interaction between fluid flow and microbial cells: importance of the operating scale
GFP Whole Cell Microbial Biosensors: Scale-up and Scale-down Effects on Biopharmaceutical Processes
List of Commercial Codes
Introduction to Finite Element, Boundary Element, and Meshless Methods: With Applications to Heat Transfer and Fluid Flow
Industrially-Relevant Multiscale Modeling of Hydrogen Assisted Degradation
International Hydrogen Conference (IHC 2012): Hydrogen-Materials Interactions