Variable thickness tube drawing is a new process for the production of high performance tubes. In this study, experiments were conducted to evaluate the effect of cross section reduction on the microstructure and mechanical properties of variable thickness aluminium tubes drawn using two different position controlled mandrel techniques. Various tubes with three different outer diameters were subjected to cold drawing at room temperature from 11% to 41% cross section reduction. The local mechanical properties were determined from tensile tests carried out on specimens cut from different positions in the tubes parallel to their axes. The distributions of the Vickers hardness over the surfaces at 0 deg and 90 deg to the drawing direction were examined. It was found that the microhardness, yield strength, and ultimate tensile of the deformed samples increase and the corresponding elongation decreases with the increase of cross section reduction. Also, the anisotropy in microstructure and mechanical properties is more significant with increasing of cross section reduction. The evolution of mechanical properties of drawn tubes versus cross section reduction depends on the mandrel shapes and initial tube outer diameter. This study helps to further understand the microstructure and mechanical properties evolutions during tube drawing process with variable thickness.