Research Papers

Formation of Highly Aligned Grooves on Inner Surface of Semipermeable Hollow Fiber Membrane for Directional Axonal Outgrowth

[+] Author and Article Information
Yu Long, Yong Huang

Department of Mechanical Engineering, Clemson University, Clemson, SC 29634

Ning Zhang, Xuejun Wen

Clemson-MUSC Bioengineering Program, Clemson University, Charleston, SC 29425

J. Manuf. Sci. Eng 130(2), 021011 (Mar 28, 2008) (8 pages) doi:10.1115/1.2896111 History: Received April 16, 2007; Revised February 03, 2008; Published March 28, 2008

It is generally believed that organized neural architecture is essential for nervous system development, function, and regeneration. In the absence of guidance cues, regenerating axons may lose their directions and become misaligned, resulting in the formation of neuromas and/or misappropriate connections. To help regenerate axons across damaged regions and guide them to appropriate targets, some bridging devices such as microgrooves are being intensively researched to achieve a better directional axonal growth. This paper reports a novel fabrication process to generate a highly aligned groove texture on the inner surface of semipermeable hollow fiber membranes (HFMs). HFMs have demonstrated promising results in guiding axonal regeneration. The fabrication process utilized a wet phase-inversion procedure with polyurethane (PU) as model polymer, dimethyl sulfoxide (DMSO) as solvent, and water as nonsolvent. Data indicated that highly aligned groove texture could be formed on the HFM inner surface by carefully controlling phase-inversion conditions such as the polymer solution flow rate, and/or nonsolvent flow rate, and/or polymer solution concentration ratio. The texture forming mechanism is qualitatively explained using a PU-DMSO-water ternary phase diagram and the dynamics of fluid instability. Axonal outgrowth on the HFM with aligned grooves showed the highly aligned orientation and improved axonal outgrowth length. This study may eventually lead to a new and effective way to fabricate nerve grafts for the spinal cord injury and nerve damage treatment based on this highly aligned three dimensional scaffold.

Copyright © 2008 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.



Grahic Jump Location
Figure 1

Schematic of the phase-inversion setup

Grahic Jump Location
Figure 2

Spinneret design (S: solvent DMSO, NS: nonsolvent water, PU: polymer polyurethane)

Grahic Jump Location
Figure 3

Typical HFM morphology with a smooth inner surface

Grahic Jump Location
Figure 4

Typical HFM morphology with an aligned groove texture on the inner surface

Grahic Jump Location
Figure 5

Approximated ternary phase diagram of the PU/DMSO/water system

Grahic Jump Location
Figure 6

Schematic of a wet phase-inversion process

Grahic Jump Location
Figure 7

Fluctuations in the solution

Grahic Jump Location
Figure 8

The match between sinusoidal wave and groove shape

Grahic Jump Location
Figure 9

HFM morphologies when polymer solution flow rates (part of Scenario 1 in the experiment design) were (a) 1.2ml∕min, (b) 1.6ml∕min, and (c) 1.8ml∕min with a constant water flow rate of 4ml∕min

Grahic Jump Location
Figure 10

Directionality of regenerated neurites on two types of HFMs. (a) Pattern of regenerated neurites on the HFMs with smooth inner surface. Neurites in most regions were not well aligned with the longitudinal axis of HFMs. (b) Neurite outgrowth pattern on the HFMs with highly aligned textures.




Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In