0
Research Papers

Development of Three-Dimensional Alginate Encapsulated Chondrocyte Hybrid Scaffold Using Microstereolithography

[+] Author and Article Information
Seung-Jae Lee

Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja dong, Nam-gu, Pohang, Kyungbuk 790-781, Korea

Jong-Won Rhie

Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-Gu, Seoul, 137-701, Korea

Dong-Woo Cho1

Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja dong, Nam-gu, Pohang, Kyungbuk 790-781, Koreadwcho@postech.ac.kr

1

Corresponding author.

J. Manuf. Sci. Eng 130(2), 021007 (Mar 20, 2008) (5 pages) doi:10.1115/1.2896114 History: Received August 19, 2007; Revised November 23, 2007; Published March 20, 2008

Hydrogels are useful materials because of their chemical similarity to extracellular matrix and their ability to rapidly diffuse hydrophilic nutrients and metabolites. Using rapid prototyping methods, we fabricated freeform three-dimensional (3D) scaffolds with chondrocytes encapsulated in an alginate hydrogel. The 3D hybrid scaffold was developed as combination of two components, a trimethylene carbonate (TMC)/trimethylolpropane (TMP) framework and an alginate hydrogel within an encapsulation of chondrocytes. To develop 3D hybrid scaffolds, we employed a microstereolithography system. The biodegradable, photopolymerizable liquid prepolymer was prepared by the polymerization of TMC with TMP and subsequently end capped with an acrylate group. The meshed framework of scaffolds withstood mechanical loading effectively. The line depth and linewidth could be controlled by varying laser power, scan path, and scan speed. Results of cell culture indicate that the biomimetic nature of these encapsulated chondrocyte scaffolds effectively retain the phenotypic function of chondrocytes within the scaffold structure. The proposed 3D hybrid scaffolds can be used for cartilage regeneration.

FIGURES IN THIS ARTICLE
<>
Copyright © 2008 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.

References

Figures

Grahic Jump Location
Figure 1

Tree diagrams representing the (a) FRs and (b) DPs

Grahic Jump Location
Figure 2

Schematic drawing of the new scaffold fabrication apparatus

Grahic Jump Location
Figure 3

Photograph of the constructed scaffold fabrication system

Grahic Jump Location
Figure 4

SEM images of fabricated microstructure using newly designed fabrication system.

Grahic Jump Location
Figure 5

Schematics of preparation process of (co)oligomer and acrylate-end-capped prepolymer and photocuring.

Grahic Jump Location
Figure 6

Experimental result of cured depth with unfocused laser beam according to scanning speed (laser power=400μW).

Grahic Jump Location
Figure 7

SEM image of TMC/TMP framework for hybrid scaffold

Grahic Jump Location
Figure 8

Schematic diagrams showing the fabrication process of 3D hybrid scaffold encapsulated cell

Grahic Jump Location
Figure 9

Images of 3D hybrid scaffold: (a) chondrocytes encapsulated in hybrid scaffold; (b) cultured chondrocytes after three days

Grahic Jump Location
Figure 10

In vivo tissue ingrowth test: (a) implantation, (b) hybrid scaffolds after four weeks

Grahic Jump Location
Figure 11

Histological evaluation of chondrocytes after four weeks implantation: (a) Alcian blue staining image (400×): (b) H&E staining image (400×)

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In