Research Papers

Development of Three-Dimensional Alginate Encapsulated Chondrocyte Hybrid Scaffold Using Microstereolithography

[+] Author and Article Information
Seung-Jae Lee

Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja dong, Nam-gu, Pohang, Kyungbuk 790-781, Korea

Jong-Won Rhie

Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-Gu, Seoul, 137-701, Korea

Dong-Woo Cho1

Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja dong, Nam-gu, Pohang, Kyungbuk 790-781, Koreadwcho@postech.ac.kr


Corresponding author.

J. Manuf. Sci. Eng 130(2), 021007 (Mar 20, 2008) (5 pages) doi:10.1115/1.2896114 History: Received August 19, 2007; Revised November 23, 2007; Published March 20, 2008

Hydrogels are useful materials because of their chemical similarity to extracellular matrix and their ability to rapidly diffuse hydrophilic nutrients and metabolites. Using rapid prototyping methods, we fabricated freeform three-dimensional (3D) scaffolds with chondrocytes encapsulated in an alginate hydrogel. The 3D hybrid scaffold was developed as combination of two components, a trimethylene carbonate (TMC)/trimethylolpropane (TMP) framework and an alginate hydrogel within an encapsulation of chondrocytes. To develop 3D hybrid scaffolds, we employed a microstereolithography system. The biodegradable, photopolymerizable liquid prepolymer was prepared by the polymerization of TMC with TMP and subsequently end capped with an acrylate group. The meshed framework of scaffolds withstood mechanical loading effectively. The line depth and linewidth could be controlled by varying laser power, scan path, and scan speed. Results of cell culture indicate that the biomimetic nature of these encapsulated chondrocyte scaffolds effectively retain the phenotypic function of chondrocytes within the scaffold structure. The proposed 3D hybrid scaffolds can be used for cartilage regeneration.

Copyright © 2008 by American Society of Mechanical Engineers
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Figure 1

Tree diagrams representing the (a) FRs and (b) DPs

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Figure 2

Schematic drawing of the new scaffold fabrication apparatus

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Figure 9

Images of 3D hybrid scaffold: (a) chondrocytes encapsulated in hybrid scaffold; (b) cultured chondrocytes after three days

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Figure 3

Photograph of the constructed scaffold fabrication system

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Figure 4

SEM images of fabricated microstructure using newly designed fabrication system.

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Figure 5

Schematics of preparation process of (co)oligomer and acrylate-end-capped prepolymer and photocuring.

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Figure 6

Experimental result of cured depth with unfocused laser beam according to scanning speed (laser power=400μW).

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Figure 7

SEM image of TMC/TMP framework for hybrid scaffold

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Figure 8

Schematic diagrams showing the fabrication process of 3D hybrid scaffold encapsulated cell

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Figure 10

In vivo tissue ingrowth test: (a) implantation, (b) hybrid scaffolds after four weeks

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Figure 11

Histological evaluation of chondrocytes after four weeks implantation: (a) Alcian blue staining image (400×): (b) H&E staining image (400×)



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