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Technical Briefs

Study of Process-Induced Cell Membrane Stability in Cell Direct Writing

[+] Author and Article Information
Jun Yin

Department of Mechanical Engineering,  Clemson University, Clemson, SC 29634

Yong Huang1

Department of Mechanical Engineering,  Clemson University, Clemson, SC 29634yongh@clemson.edu

http://moose.bio.ucalgary.ca/

1

Corresponding author.

J. Manuf. Sci. Eng 133(5), 054501 (Aug 30, 2011) (5 pages) doi:10.1115/1.4004685 History: Received September 27, 2009; Revised July 17, 2011; Published August 30, 2011; Online August 30, 2011

Process-induced damage to cells is of significant importance and must be mitigated for safe and reproducible cell direct writing. The objective of this study is to investigate the cell membrane stability under the external normal pressure. This investigation is performed by studying the dipalmitoylphosphatidylcholine bilayer behavior under different normal pressures using molecular dynamics. As the normal pressure increases, the force necessary to rupture the bilayer structure decreases, which indicates cell membrane instability under high normal pressure. This phenomenon can also be explained by the change of free energy difference before and after rupture under different normal pressures. The effect of the pulling speed on the rupture force is also investigated, showing that the rupture force increases almost linearly with the pulling speed.

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Copyright © 2011 by American Society of Mechanical Engineers
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Figures

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Figure 1

Laser-assisted cell direct-write setup and MD simulation model

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Figure 2

DPPC bilayer thickness variation under different normal pressures

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Figure 3

(a) Average DPPC bilayer thickness and average area of per DPPC molecule and (b) average rupture force under different normal pressures

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Figure 4

Average rupture force under different pulling speeds

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Figure 5

(a) Free energy difference before and after rupture, and (b) relationship between the free energy difference and the applied normal pressure

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